Demonstration of percellular channels in glamerular podocytes in aminonucleoside nephosis.

Nephrotic syndrome was experimentally induced by a single i.v. injection of puromyein. Permeability of the visceral epithelial wall of glomeruli was examined with electron microscope. 30-60 seconds prior to fixation, ferritin solution was injected into the urinary space of superficially located glomeruli either by direct micropuncture of the urinary space or by retrograde filling of the urinary space via the corresponding tubule. Experimental evidence is presented for formation of channel-like pathways of ferritin penetration through the epithelial layer. Since these structures in nephrotic glomeruli are surrounded by a single cell only, we would like to propose the term percellular channel for this phenomenon.